1887

Abstract

Three Gram-stain-negative, aerobic, motile and rod-shaped bacterial strains, 7Q-K02, DHF22 and DHOM02, were isolated from forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, China. Strains 7Q-K02, DHF22 and DHOM02 grew at 4–37, 4–42 and 12–37 °C, pH 3.0–8.5, 3.5–8.5 and 5.0–8.0, and in the presence of 0–3.0, 0–3.5 and 0–2.5 % (w/v) NaCl; with optima at 28–33, 28 and 28–33 °C, pH 3.5–6.5, 4.0–5.5 and 6.5–7.0, and 0–1.5, 0–1.5 and 0.5–1.5 % (w/v) NaCl, respectively. Strains 7Q-K02 and DHF22 have the highest 16S rRNA gene sequence similarities of 99.0 and 98.0 % to LMG 19450 and 97.7 % between themselves, while strain DHOM02 shares the highest similarity of 98.4 % to ‘ A396 followed by 98.3 % to ATCC 43733. In the 16S rRNA gene sequence phylogram, strain 7Q-K02 formed a sister branch with , and , and strain DHF22 was separated from all other species within the genus , while strain DHOM02 formed a separated clade with members of the genus . The DNA G+C contents of strains 7Q-K02, DHF22 and DHOM02 wwe 64.3, 65.4 and 66.6 %, respectively. Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values of strains 7Q-K02, DHF22 and closely related strains were in the ranges of 25.5–43.7 % and 81.5–91.3 %, respectively. While dDDH and ANI values between strain DHOM02 and strains with genome sequence data were in the ranges of 22.4–31.0 % and 78.2–86.1 %, respectively. These three strains have the same major respiratory quinone: ubiquinone-8. Strains 7Q-K02, DHF22 and DHOM02 have C, C cyclo, C cyclo 8 and summed feature 8 (C 7/C 6) as their major fatty acid compositions. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of phenotypic, phylogenetic, genomic analyses and chemotaxonomic data, strains 7Q-K02 and DHF22 represent two novel species of the genus , for which the names sp. nov. (type strain 7Q-K02=CGMCC 1.15433=KCTC 62472=LMG 29209) and sp. nov. (type strain DHF22=GDMCC 1.1448=LMG 30262) are proposed, while strain DHOM02 represents a novel species in the genus , for which the name sp. nov. (type strain DHOM02=KCTC 42625=LMG 28843) is proposed. We also propose to transfer to the genus as comb. nov. based mainly on the results of phylogenomic analysis.

Funding
This study was supported by the:
  • Guangdong Province Science and Technology Innovation Strategy Special Fund (Award 2018B020205003)
    • Principle Award Recipient: Li-hongQiu
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2021-02-08
2021-10-25
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