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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., isolated from phenanthrene-degrading consortium enriched from Antarctic soil Free

Abstract

A novel bacterium, designated strain ANT13_2, was isolated from a phenanthrene-degrading consortium enriched from a soil sample collected near the Great Wall Station located in the southwestern area of King George Island, Antarctica. Following a polyphasic taxonomic study, a novel species belonging to the genus was described. The strain was a Gram-stain-positive bacterium that exhibited a rod–coccus growth cycle. Strain ANT13_2 grew aerobically at an optimum temperature of 20–25 °C and at pH 7.0–8.0. Ribose, arabinose and glucose were detected as whole-cell sugars. The predominant menaquinone was MK-9. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The predominant cellular fatty acids were anteiso-C (67.7 %) and anteiso-C (11.2 %). The DNA G+C content of the genomic DNA was 60.6 mol%. Based on 16S rRNA gene sequence analysis, strain ANT13_2 showed the highest similarities to SPC26 (98.9 %) followed by Lz1y (98.4 %), DSM 20167 (98.3%) and KGN15 (97.9 %). The average nucleotide identity values between strain ANT13_2 and the type strains of SPC26 and Lz1y were 73.8 and 77.5 %, respectively, which are well below the 95–96 % species circumscription threshold. On the basis of this polyphasic taxonomic study, strain ANT13_2 is proposed to represent a novel species to be named sp. nov. The type strain is ANT13_2 (=TBRC 11756=NBRC 114615).

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  • Accepted:
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Keyword(s): actinomycete , Antarctica , Paeniglutamicibacter terrestris and phenanthrene-degrading consortium
Funding
This study was supported by the:
  • Ratchadapisek Somphot Fund for Postdoctoral Fellowship, Chulalongkorn University.
    • Principle Award Recipient: ChatsudaSakdapetsiri
  • Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Thailand
    • Principle Award Recipient: OnruthaiPinyakong
  • Technology Development Agency, Chinese Arctic and Antarctic Administration
    • Principle Award Recipient: ChenBo
  • the Information Technology Foundation under the Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn, Polar Research Project under the Initiatives of Her Royal Highness Princess Maha Chakri Sirindhorn, National Science
    • Principle Award Recipient: OnruthaiPinyakong
© 2021 The Authors
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2021-02-08
2024-04-18
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Paeniglutamicibacter terrestris sp. nov., isolated from phenanthrene-degrading consortium enriched from Antarctic soil
Int J Syst Evol Microbiol 71, 004689 (2021); https://doi.org/10.1099/ijsem.0.004689
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