1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., isolated from the hadal seawater of the Mariana Trench Free

Abstract

A Gram-staining-negative, strictly aerobic, long-rod shaped with no flagellum and yellow-pigmented bacterium designated strain ZXX205, was isolated from the hadal seawater at the depth of 7500 m in the Mariana Trench, Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences placed strain ZXX205 within the genus and strain ZXX205 was most closely related to KCTC 52348 and KCTC 22026 with 96.9 % and 96.6 % sequence similarity, respectively. The sequence similarities to all other type strains were 96.3 % or less, and to the type strain LMG 22492 was 94.1 %. Growth occurred in the presence of 0–9.0 % (w/v) NaCl (optimum 3.0 %), at 4–45 °C (optimum 28 °C) and pH 6.0–9.0 (optimum pH 7.5). The sole respiratory quinone was menaquinone 6 (MK-6). The dominant cellular fatty acids (>10 %) of strain ZXX205 were iso-C, iso-C G, iso-C 3-OH and iso-C. The polar lipids profile contained predominantly phosphatidylethanolamine, four glycolipids, four unidentified aminolipids and three unidentified lipids. The genomic DNA G+C content was 35.5 %. The DNA–DNA relatedness (DDH) values between strain ZXX205 and the most closely related species and were 21.1 and 20.4 %, respectively. Based on polyphasic taxonomic analysis, strain ZXX205 is considered to represent a novel species in the genus of the family , for which the name is proposed. The type strain is ZXX205 (=MCCC 1K03851=JCM 33665).

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  • Accepted:
  • Published Online:
Keyword(s): hadal seawater , Mariana Trench and Winogradskyella ouciana sp. nov
Funding
This study was supported by the:
  • Fundamental Research Funds for Central Universities of the Central South University (Award Nos. 201762009 and 201762017)
    • Principle Award Recipient: Xiao-HuaZhang
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award Nos. 91751202 and 41730530)
    • Principle Award Recipient: Xiao-HuaZhang
© 2021 The Authors
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2021-02-08
2024-04-24
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Winogradskyella ouciana sp. nov., isolated from the hadal seawater of the Mariana Trench
Int J Syst Evol Microbiol 71, 004687 (2021); https://doi.org/10.1099/ijsem.0.004687
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