@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004673, author = "Rasmussen, Torben Sølbeck and Streidl, Theresa and Hitch, Thomas C. A. and Wortmann, Esther and Deptula, Paulina and Kofoed, Michael V. W. and Riedel, Thomas and Neumann-Schaal, Meina and Hansen, Michael and Nielsen, Dennis Sandris and Clavel, Thomas and Vogensen, Finn Kvist", title = "Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2021", volume = "71", number = "3", pages = "", doi = "https://doi.org/10.1099/ijsem.0.004673", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004673", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "acetate", keywords = "spore-forming", keywords = "succinate", keywords = "mouse faeces", keywords = "diet-induced obesity", keywords = "Firmicutes", keywords = "Lachnospiraceae", eid = "004673", abstract = "A bacterial strain, designated WCA-9-b2T, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order Clostridiales with Dorea longicatena ATCC 27755T (94.9 % sequence identity), Ruminococcus gnavus ATCC 29149T (94.8%) and Clostridium scindens ATCC 35704T (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52–74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives ( D. longicatena , Ruminococcus gnavus , C. scindens , Dorea formicigenerans , Ruminococcus lactaris , Clostridium hylemonae , Merdimonas faecis , Faecalicatena contorta and Faecalicatena fissicatena ). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C16 : 0 (24.5%), C18 : 1 cis9 (19.8 %), C16 : 0 DMA (11.7%), C18 : 0 (8.4%) and C14 : 0 (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO2 and H2 were detected. Based on these data, we propose that strain WCA-9-b2T represents a novel species within a novel genus, for which the name Sporofaciens musculi gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2T (=DSM 106039T=CECT 30156T).", }