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Volume 71, Issue 3

sp. nov., isolated from a marine Mediterranean sponge Free

Abstract

A Gram-stain-positive bacterium, strain RV15, forming an extensively branched substrate mycelium and aerial hyphae that differentiate into spiral chains of spores, was isolated from a marine sponge collected from Rovinj (Croatia). Comparison of 16S rRNA gene sequences showed that strain RV15 is a member of the genus with highest sequence similarity to the type strains of (98.8 %) (98.6 %) and (98.5 %). Sequence similarities to all other types strains were below 98.5 %. The multilocus sequence analysis-based evolutionary distance, the average nucleotide identity value and the genome-to-genome distance of strain RV15 and the type strain of were clearly below the species cut-off values. Strain RV15 exhibited a quinone system composed of the major menaquinones MK-9(H), MK-9(H) and MK-9(H), typical for the genus . The polar lipid profile of strain RV15 consisted of the predominant compounds diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of phosphatidylinositol, phosphatidylinositol mannoside, an unidentified lipid and an unidentified phospholipid. Major polyamines were spermine and spermidine. The diagnostic diaminoacid of the peptidoglycan was -diaminopimelic acid. The major fatty acids were iso C, anteiso C ω9 and anteiso C. The results of physiological and biochemical tests allowed further phenotypic differentiation of strain RV15 from its most-related species and hence clearly merits species status. We propose the name sp. nov. with the type strain RV15 (=DSM 42110=LMG 27702).

  • Received:
  • Accepted:
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Keyword(s): Acintobacteria , ANI , core genome phylogeny , sponge , Streptomyces and taxonomy
© 2021 The Authors
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2021-01-29
2024-04-23
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Streptomyces dysideae sp. nov., isolated from a marine Mediterranean sponge Dysidea tupha
Int J Syst Evol Microbiol 71, 004672 (2021); https://doi.org/10.1099/ijsem.0.004672
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