1887

International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 2

sp. nov. a novel actinobacterium isolated from soil No Access

Abstract

A novel actinobacterium, designated strain NEAU-D13, was isolated from soil collected from Aohan Banner, Chifeng, Inner Mongolia Autonomous Region, China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-D13 belonged to the genus and shared the highest sequence similarity with JCM 14913 (99.17 %). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus . Cell walls contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were ribose and mannose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylinositol. The menaquinone was only MK-9(H). The major fatty acids were -C, C, -C, -C and -C. DNA G+C content was 68.71 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a stable clade with JCM 14913 in the genus . Meanwhile, a combination of digital DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-D13 could be distinguished from its closely related strain. Therefore, it is concluded that strain NEAU-D13 represents a novel species of the genus , for which the name sp. nov. is proposed, with NEAU-D13 (=CCTCC AA 2019089=JCM 33970) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Genome , Lentzea alba sp. nov. and Polyphasic taxonomy
Funding
This study was supported by the:
  • “Academic Backbone” Project of Northeast Agricultural University (Award 19XG18)
    • Principle Award Recipient: JiaSong
  • Heilongjiang Provincial Postdoctoral Science Foundation (Award LBH-Z15016)
    • Principle Award Recipient: JiaSong
  • China Postdoctoral Science Foundation (Award 2015M580255)
    • Principle Award Recipient: JiaSong
  • National Natural Science Foundation of China (Award 31700067)
    • Principle Award Recipient: JiaSong
© 2021 The Authors
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2021-10-17
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Lentzea alba sp. nov., a novel actinobacterium isolated from soil
Int J Syst Evol Microbiol 71, 004661 (2021); https://doi.org/10.1099/ijsem.0.004661
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