1887

Abstract

Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10 and 4HC-13, were isolated from the faeces of greater white-fronted geese () at Poyang Lake, Jiangxi Province, PR China. Optimal growth was observed at 35–37 °C, pH 7.0–8.0 and with 0.5–1.5 % (w/v) NaCl. The 16S rRNA gene sequences of strains 23H37-10 and 4HC-13 were identical. Phylogenetic and phylogenomic analyses indicated that strains 23H37-10 and 4HC-13 formed an independent cluster within the genus and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to LMM 1652, DSM 7109, DSM 44353 and NCTC 11913, respectively. Cells contained C 9, C and C as the major cellular fatty acids and MK-9 (H) as the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. Strain 23H37-10 contained mycolic acids, with -diaminopimelic acid and arabinose as the major whole-cell hydrolysates. The genome G+C content of strains 23H37-10 and 4HC-13 was 55.2 mol%. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10 and 4HC-13 were 94.4 and 99.6 %, respectively. Strains 23H37-10 and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus , respectively. The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10 and 4HC-13 represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 23H37-10 (=GDMCC 1.1737=KACC 21672).

Funding
This study was supported by the:
  • Independent Research Project of State Key Laboratory of Infectious Disease Prevention and Control (Award 2019SKLID311)
    • Principle Award Recipient: ShengenChen
  • the Major Science Project of Jiangxi Province (Award 2020BBG71010)
    • Principle Award Recipient: ChenShengen
  • the National Science and Technology Major Projects (Award 2018ZX10201001-006)
    • Principle Award Recipient: XiongYanwen
  • the National Science and Technology Major Project (Award 2018ZX10301407-002)
    • Principle Award Recipient: XiongYanwen
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2021-01-11
2021-07-29
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