1887

Abstract

An aerobic, rod-shaped, Gram-stain-positive, actinobacterial strain, designated 1.0914, was isolated from a stalactite sample collected from a cave located in Guizhou Province, southwest PR China. Based on 16S rRNA gene sequence analysis, strain 1.0914 shared highest similarities values with CGMCC 4.7388 (97.7 %), CCTCC AB 2017083 (97.5 %) and CCTCC AB 2018079 (97.3 %) and values lower than 97.0 % to other members of the genus . Phylogenetic trees based on 16S rRNA gene sequences indicated that strain 1.0914 formed an isolated branch with CGMCC 4.7388, CCTCC AB 2017083 and CCTCC AB 2018079. The polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid in the cellular membrane. The major fatty acids were identified as iso-C, C ω9, C ω8 and C. The predominant respiratory quinone was MK-8(H) and -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The genomic DNA G+C content was 71.1 mol%. The orthologous average nucleotide identiy values between CGMCC 4.7388, CCTCC AB 2017083, CCTCC 2018079 and strain 1.0914 were 82.3, 81.7 and 81.9 % respectively. DNA–DNA hybridization values between CGMCC 4.7388, CCTCC AB 2017083, CCTCC 2018079 and strain 1.0914 were 25.2, 24.6 and 24.5 % respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of strain 1.0914 as representing a new species of , for which the name sp. nov. is proposed. The type strain is 1.0914 (=CCTCC AB 2018266=KCTC 49243).

Funding
This study was supported by the:
  • the Department of Science and Technology of Yunnan Province (Award 202001BB050072)
    • Principle Award Recipient: He MoMing
  • the Department of Science and Technology of Yunnan Province (Award 2019ZG00901)
    • Principle Award Recipient: He MoMing
  • the Department of Science and Technology of Yunnan Province (Award 2018BC001)
    • Principle Award Recipient: MingHe Mo
  • China Tobacco Yunnan Industrial Co. Ltd. (Award 2019530000241018)
    • Principle Award Recipient: MingHe Mo
  • China Tobacco Yunnan Industrial Co. Ltd. (Award 2018530000241019)
    • Principle Award Recipient: MingHe Mo
  • the National Natural Science Foundation of China (Award 31660023)
    • Principle Award Recipient: He MoMing
  • the National Natural Science Foundation of China (Award 31870091)
    • Principle Award Recipient: MingHe Mo
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