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Volume 71, Issue 2

sp. nov., isolated from a tidal flat sediment and proposal for the reclassification of as comb. nov. Free

Abstract

A novel Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile bacterial strain, designated as 4C16A, was isolated from a tidal flat sediment and characterized by using a polyphasic taxonomic approach. Strain 4C16A was found to grow at 10–40 °C (optimum, 28 °C), at pH 5.0–10.0 (optimum, pH 6.0–7.0) and in 0–6 % (w/v) NaCl (optimum, 1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 4C16A fell into the genus , and shared the highest identity of 98.9 % with the closest type strain KACC 13772 and less than 98.0 % identity with other type strains of recognized species within this genus. The phylogenomic analysis indicated that strain 4C16A formed an independent branch within this genus. The 28.6 % digital DNA–DNA hybridization estimate and 85.0 % average nucleotide identity between strains 4C16A and KACC 13772 were the highest, but still far below their respective threshold for species definition, implying that strain 4C16A should represent a novel genospecies. The predominant cellular fatty acid was summed feature 8; the polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmonomethylethanolamine; the respiratory quinones were Q-9 and Q-10. The genomic DNA G+C content was 59.8mol %. Based on phylogenetic analyses and phenotypic and chemotaxonomic characteristics, strain 4C16A is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the species is 4C16A (=GDMCC 1.1932=KACC 22078). We also propose the reclassification of as comb. nov. and ‘’ as sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genomic analysis , genotypes and phenotypes , polyphasic taxonomy and Roseibium litorale
Funding
This study was supported by the:
  • The Science and Technology Plan Project of Guangdong Province (Award 2019B030316017)
    • Principle Award Recipient: Ming-RongDeng
  • The GDAS' Project of Science and Technology Development (Award 2020GDASYL-20200103017)
    • Principle Award Recipient: YangLiu
  • The Key-Area Research and Development Program of Guangdong Province (Award 2018B020205003)
    • Principle Award Recipient: HonghuiZhu
© 2021 The Authors
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2021-01-12
2024-04-25
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Roseibium litorale sp. nov., isolated from a tidal flat sediment and proposal for the reclassification of Labrenzia polysiphoniae as Roseibium polysiphoniae comb. nov.
Int J Syst Evol Microbiol 71, 004634 (2021); https://doi.org/10.1099/ijsem.0.004634
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