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Abstract

A Gram-stain-negative, aerobic, short rod-shaped, pale yellow-pigmented, non-motile and gentamycin-resistant bacterial strain designated CJ210 was isolated from the Han River, Republic of Korea. Strain CJ210 grew optimally at 30 °C and pH 7.0 in the absence of NaCl on tryptic soy agar. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain CJ210 belonged to the genus within the family and was most closely related to KACC 14347 (98.1 % similarity), followed by KCTC 23367 (95.3 % similarity). The average nucleotide identity values between strain CJ210 and two closely related type strains KACC 14347 and KCTC 23367 were 83.7 and 73.8 %, respectively. The digital DNA–DNA hybridization results between strain CJ210 and the related type strains were 27.5 and 20.2 %, respectively. Strain CJ210 contained menaquinone 6 (MK-6) as the predominant menaquinone. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The major fatty acids of strain CJ210 were iso-C, iso-C 3-OH and summed feature 9 (comprising iso-C 9 and/or C 10-methyl). Whole genome sequencing revealed that strain CJ210 had a genome of 3.8 Mbp with 36.5 % DNA G+C content. The genome contained several antimicrobial resistance genes including an aminoglycoside-resistant gene. On the basis of the polyphasic taxonomic study, strain CJ210 represents a novel species in the genus , for which name sp. nov. is proposed. The type strain is CJ210 (=KACC 19954=JCM 33306).

Funding
This study was supported by the:
  • Korea Environmental Industry and Technology Institute (Award 2016001350004)
    • Principle Award Recipient: Chang-JunCha
  • National Institute of Biological Resources (Award NIBR201801204)
    • Principle Award Recipient: Chang-JunCha
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2021-01-19
2021-10-18
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