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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 2

gen. nov., sp. nov., a member of the family , isolated from a saline and alkaline lake sediment Free

Abstract

A non-motile, Gram-staining negative, catalase- and oxidase-positive, crescent-rod shaped bacterium, designated strain CUG 91308, was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. Colonies on OSM agar were round, smooth, flat and pinkish-orange in colour. Strain CUG 91308 could grow at 15–37 °C, pH 6–12 and in the presence of up to 7.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91308 belonged to the family and formed a clade with the genus in the phylogenetic tree, but separated from any species of the known genera within the family. The genomic DNA G+C content is about 42.1 %. The predominant fatty acids (>10 %) were iso-C (21.1 %), summed feature 3 (C  7c / C  6c / iso-C 2OH) (14.3 %), iso-C 3OH (12.3 %) and summed feature 9 (iso-C  9c / C 10-methyl) (10.6 %). The polar lipids of strain CUG 91308 were phosphatidylethanolamine (PE) and four unidentified polar lipids. Strain CUG 91308 contained MK-7 as the major respiratory quinone. On the basis of phenotypic, genotypic and phylogenetic data, strain CUG 91308 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the proposed new isolate is CUG 91308 (=KCTC 62636=CGMCC 1.13593).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cyclobacteriaceae , Lunatibacter salilacus gen. nov.sp. nov , Polyphasic taxonomy , Qinghai Lake and sediment
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 91751206)
    • Principle Award Recipient: HongchenJiang
© 2021 The Authors
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2021-01-06
2025-04-29
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Lunatibacter salilacus gen. nov., sp. nov., a member of the family Cyclobacteriaceae, isolated from a saline and alkaline lake sediment
Int J Syst Evol Microbiol 71, 004621 (2021); https://doi.org/10.1099/ijsem.0.004621
/content/journal/ijsem/10.1099/ijsem.0.004621
/content/journal/ijsem/10.1099/ijsem.0.004621
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