1887

Abstract

A moderately halophilic, Gram-stain-negative, aerobic bacterium, strain D1-1, belonging to the genus , was isolated from soil sampled at Pentha beach, Odisha, India. Phylogenetic trees reconstructed based on 16S rRNA genes and multilocus sequence analysis of and genes revealed that strain D1-1 belonged to the genus and was most closely related to YKJ-16 (98.1 %) followed by Al12 (97.5 %), CPS11 (97.5 %), 5CR (97.4 %) and DSM 735 (97.2 %) on the basis of 16S rRNA gene sequence similarity. Sequence identities with other species within the genus were lower than 97.0 %. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values of 22.4–30 % and 79.5–85.4 % with close relatives of DSM 735, . YKJ-16, Al12 and 5CR were lower than the threshold recommended for species delineation (70 % and 95–96 % for dDDH and ANI, respectively). Further, strain D1-1 formed yellow-coloured colonies; cells were rod-shaped, motile with optimum growth at 30 °C (range, 4–45 °C) and 2–8 % NaCl (w/v; grew up to 24 % NaCl). The major fatty acids were summed feature 8 (C 7/C 6), summed feature 3 (C 7/C 6) and C and the main respiratory quinone was ubiquinone Q-9 in line with description of the genus. Based on its chemotaxonomic and phylogenetic characteristics and genome uniqueness, strain D1-1 represents a novel species in the genus , for which we propose the name sp. nov., within the family . The type strain is D1-1 (=JCM 33602=KACC 21317=NAIMCC-B-2254).

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2020-12-22
2021-03-01
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