1887

Abstract

Three bacterial strains, designated Red330, Red736 and Red745, were isolated from forest and paddy soils in Japan. Strains Red330, Red736 and Red745 are flagella-harbouring and strictly anaerobic bacteria forming red colonies. A 16S rRNA gene sequence-based phylogenetic tree showed that all three strains were located in a cluster, including the type strains of species, which were recently separated from the genus within the family . Similarities of the 16S rRNA gene sequences among the three strains and S43, the type species of the genus , were 96.3–98.5 %. The genome-related indexes, average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity, among the three strains and e S43 were 74.7–86.8 %, 21.2–33.3 % and 70.4–89.8 %, respectively, which were lower than the species delineation thresholds. Regarding the phylogenetic relationships based on genome sequences, the three strains clustered with the type strains of species, which were independent from the type strains of species. The distinguishableness of the three isolated strains was supported by physiological and chemotaxonomic properties, with the profile of availability of electron donors and cellular fatty acids composition being particularly different among them. Based on genetic, phylogenetic and phenotypic properties, the three isolates represent three novel independent species in the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are Red330 (=NBRC 114028=MCCC 1K03949), Red736 (=NBRC 114029=MCCC 1K03950) and Red745 (=NBRC 114030=MCCC 1K03951), respectively.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP18K14366)
    • Principle Award Recipient: YokoMasuda
  • Japan Society for the Promotion of Science (Award JP18K19165)
    • Principle Award Recipient: KeishiSenoo
  • Japan Society for the Promotion of Science (Award JP18K19165)
    • Principle Award Recipient: HideomiItoh
  • Japan Society for the Promotion of Science (Award JP17H01464)
    • Principle Award Recipient: KeishiSenoo
  • Japan Society for the Promotion of Science (Award JP17H01464)
    • Principle Award Recipient: HideomiItoh
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/content/journal/ijsem/10.1099/ijsem.0.004607
2020-12-09
2021-10-17
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