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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 1

sp. nov., isolated from an Antarctic marine sponge Free

Abstract

A Gram-negative, aerobic, rod-shaped, non-motile bacterium, designated strain HQ09, was isolated from a marine sponge off the coast of Fields Peninsula, West Antarctica. Strain HQ09 grew at 4–35 °C (optimum, 25 °C), pH 5–9 (optimum, pH 7.0), and with 1–10% NaCl (optimum, 2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain HQ09 was affiliated with the genus in the family , sharing 99.64 % identity with the type strain of , the only known species in the genus. However, the low digital DNA–DNA hybridization (dDDH) (27.2 %) and average nucleotide identity (ANI) (83.63 %) values between strain HQ09 and the type strain of indicated that they did not belong to the same species. Strain HQ09 could also be differentiated from by many phenotypic characteristics. The major fatty acids (>5 %) of strain HQ09 were summed feature 8 (C C ω6c), 11-methyl C c, C and C19 : 0 cyclo 8. The polar lipids included phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and one unidentified phospholipid. The predominant respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content was 62.63 mol%. Four secondary metabolite biosynthetic gene clusters were detected in the genome, potentially producing ectoine and three types of unknown compounds. On the basis of the polyphasic evidences obtained in this study, strain HQ09 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain being HQ09 (=KCTC 52229=CGMCC 1.15538).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Antarctic , Pseudopuniceibacterium antarcticum sp. nov , Rhodobacteraceae and Sponge
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41976224)
    • Principle Award Recipient: LiLiao
  • National Key Research and Development Program of China (Award 2018YFC1406701)
    • Principle Award Recipient: BoChen
  • National Key Research and Development Program of China (Award 2018YFC1406702)
    • Principle Award Recipient: LiLiao
© 2021 The Authors
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2020-12-17
2024-12-08
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Pseudopuniceibacterium antarcticum sp. nov., isolated from an Antarctic marine sponge
Int J Syst Evol Microbiol 71, 004605 (2020); https://doi.org/10.1099/ijsem.0.004605
/content/journal/ijsem/10.1099/ijsem.0.004605
/content/journal/ijsem/10.1099/ijsem.0.004605
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