1887

Abstract

A Gram-stain-negative, rod-shaped, mesophilic, milky white-pigmented, aerobic, non-spore-forming and non-flagellated bacterium, designated strain X16, was isolated from urban soil of Zibo, Shandong, China. According to 16S rRNA gene sequence analysis, the isolate showed highest similarities with 5GH32-13 (97.6 %), KIS20-3 (96.2 %), Gsoil 221 (96.0 %) and T16R-265 (95.8 %). The neighbour-joining tree based on 16S rRNA gene sequences showed that strain X16 formed a subcluster with 5GH32-13, and the subcluster was closely related to KIS20-3, Gsoil 221 and T16R-265. Strain X16 also formed a subcluster with 5GH32-13 in phylogenetic tree based on genomic sequences. The polar lipids are phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids, two unknown lipids and two unknown phospholipids. The major quinone of strain X16 is menaquinone-7 and the main fatty acids (>10 % of total fatty acids) of strain X16 are iso-C, iso-C 3-OH and iso-C G. The genome length of strain X16 is 8.7 Mb with a DNA G+C content of 47.4 %. ANI values among strain X16 and strain 5GH32-13, Gsoil 221, and T16R-265 are 78.1, 70.7, 70.6 %, respectively. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain X16 represents a novel species. Besides, strain X16 can detoxify high toxicity selenite [Se(IV)] to low toxicity elemental selenium [Se(0)], for which the name sp. nov. is proposed. The type strain is X16 (=KACC 21698=GDMCC1.1757).

Funding
This study was supported by the:
  • Innovative Research Group Project of the National Natural Science Foundation of China (CN) (Award 31971197)
    • Principle Award Recipient: LijuanWang
  • National Natural Science Foundation of China (Award 81602962)
    • Principle Award Recipient: FengSang
  • National Natural Science Foundation of China (Award 31500085)
    • Principle Award Recipient: HongliangLiu
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2020-12-04
2021-07-29
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