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Abstract

A haloalkaliphilic strain XQ-INN 246 was isolated from the sediment of a salt pond in Inner Mongolia Autonomous Region, China. Cells of the strain were rods, motile and strictly aerobic. The strain was able to grow in the presence of 2.6–5.3 M NaCl (optimum concentration is 4.4 M) at 30–50 °C (optimum temperature is 42 °C) and pH 7.0–10.0 (optimum pH is 8.0–8.5). The whole genome sequencing of strain XQ-INN 246 revealed a genome size of 4.52 Mbp and a DNA G+C content of 62.06 mol%. Phylogenetic tree based on 16S rRNA gene sequences and concatenated amino acid sequences of 122 single-copy conserved proteins revealed a robust lineage of the strain XQ-INN 246 with members of related genera of the family . The strain possessed the polar lipids of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Based on phylogenetic analysis, phenotypic characteristics, chemotaxonomic properties and genome relatedness, the isolate was proposed as the type strain of a novel species of a new genus within the family for which the name gen. nov., sp. nov. is proposed. The type strain is XQ-INN 246 (=CGMCC 1.16692=JCM 33751).

Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Project (Award 2017FY100300)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 91751201)
    • Principle Award Recipient: HuaXiang
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2020-12-04
2021-07-29
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