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Volume 71, Issue 1

Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov. Free

Abstract

Five strains, VB23, VB24, VB25, VB26 and VB31, were isolated from chimpanzee (), cotton-top tamarin (), Goeldi’s marmoset (), moustached tamarin () and patas monkey (), respectively, which were kept in two Czech zoos. These strains were isolated from faecal samples and were Gram-positive, non-motile, non-sporulating, anaerobic and fructose-6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA revealed close relatedness between VB23 and LMG 11039 (96.0 %), VB24 and subsp. DSM 20433 (96.1 %), VB25 and LMG 30939 (96.5 %), VB26 and LMG 30297 (98.1 %), and VB31 and . LMG 11039 (99.40 %). Internal transcribed spacer profiling revealed that VB23, VB24, VB25, VB26 and VB31 had highest similarity to LMG 13208 (77.2 %), subsp. ATCC 15697 (85.8 %), DSM 23969 (76.9 %), LMG 13208 (81.2 %) and LMG 11039 (88.2 %), respectively. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) analyses with their closest neighbours supported the independent phylogenetic positions of the strains with values between 86.3 and 94.3 % for ANI and 25.8 and 54.9 % for dDDH. These genomic and phylogenetic analyses suggested that the evaluated strains were novel species named sp. nov. (VB31=DSM 109960=CCUG 73843), sp. nov. (VB25=DSM 109958=CCUG 73842), sp. nov. (VB24=DSM 109957=CCUG 73932), sp. nov. (VB26=DSM 109959=CCUG 73845) and sp. nov. (VB23=DSM 109963=CCUG 73840).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bifidobacterium , captivity , FAMEs , monkeys , phylogenomics and taxonomy
Funding
This study was supported by the:
  • MŠMT ČR (Award No. CZ.02.1.01/0.0/0.0/16_019/0000845))
    • Principle Award Recipient: Not Applicable
  • MŠMT ČR (Award 8J19AT028)
    • Principle Award Recipient: Vera Neuzil-Bunesova
© 2021 The Authors
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2020-11-23
2024-04-19
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Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: Bifidobacterium erythrocebi sp. nov., Bifidobacterium moraviense sp. nov., Bifidobacterium oedipodis sp. nov., Bifidobacterium olomucense sp. nov. and Bifidobacterium panos sp. nov.
Int J Syst Evol Microbiol 71, 004573 (2020); https://doi.org/10.1099/ijsem.0.004573
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/content/journal/ijsem/10.1099/ijsem.0.004573
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