1887

Abstract

Six aerobic, non-motile, non-haemolytic, Gram-stain-negative, oxidase-negative strains (185, 187, 323-1, 194, dk386 and dk771) were recovered from different faecal samples of on the Qinghai–Tibet Plateau. In the 16S rRNA gene sequences, one strain pair, 185/187, shared highest similarity to 114 (97.9 %), and the other two (323-1/194 and dk771/dk386) to CGMCC 1.12528 (98.6 and 97.0 %, respectively). Phylogenomic tree analysis showed that these six strains formed three separate clades in the genus . Digital DNA–DNA hybridization values of each pair of the isolates with all members of the genus were far below 70 %. The main cellular fatty acids of all six strains were C 9, C and summed feature 3 (C 7/C 6). Q-9 was the predominant respiratory quinone for strains 185, 323-1 and dk386. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the genotypic, phenotypic and biochemical analyses, these six strains represent three novel species of the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are 185 (=CGMCC 1.13636=JCM 33607), 323-1 (=CGMCC 1.13940=JCM 33608) and dk386 (=CGMCC 1.16589=JCM 33592), respectively.

Funding
This study was supported by the:
  • Jianguo Xu , Research Units of Discovery of Unknown Bacteria and Function, Chines Academy of Medical Sciences , (Award 2018RU010)
  • Jianguo Xu , Sanming Project of Medicine in Shenzhen , (Award SZSM201811071)
  • Shan Lu , National Science and Technology Major Project of China , (Award 2018ZX10712001-018)
  • Not Applicable , National Science and Technology Major Project of China , (Award 2018ZX10712001-017)
  • Jing Yang , National Science and Technology Major Project of China , (Award 2018ZX10712001-007)
  • Jing Yang , National Key R&D Program of China , (Award 2019YFC1200505)
  • Jing Yang , National Key R&D Program of China , (Award 2019YFC1200500)
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2020-11-16
2021-02-26
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