RT Journal Article SR Electronic(1) A1 Guo, Qiannan A1 Wang, Bo A1 Zhou, Zhengfu A1 Ke, Xiubin A1 Zhang, Lijuan A1 Chen, Ming A1 Lin, Min A1 Wang, Wei A1 Zhang, Wei A1 Li, XufengYR 2020 T1 Ornithinimicrobium pratense sp. nov., isolated from meadow soil JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 12 SP 6450 OP 6457 DO https://doi.org/10.1099/ijsem.0.004557 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %). However, the digital DNA–DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium pratense sp. nov. is proposed. The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004557