%0 Journal Article %A Guo, Qiannan %A Wang, Bo %A Zhou, Zhengfu %A Ke, Xiubin %A Zhang, Lijuan %A Chen, Ming %A Lin, Min %A Wang, Wei %A Zhang, Wei %A Li, Xufeng %T Ornithinimicrobium pratense sp. nov., isolated from meadow soil %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 12 %P 6450-6457 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.004557 %K polyphasic approach %K Ornithinimicrobium %K meadow soil %K whole-genome sequencing %I Microbiology Society, %X A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %). However, the digital DNA–DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium pratense sp. nov. is proposed. The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004557