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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., isolated from meadow soil Free

Abstract

A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204 was closely related to CPCC 203535 (97.4 %, similarity), CGMCC 4.5582 (96.9 %), GP-T3-3 (96.8 %), JLT9 (96.7 %), CPCC 203383 (96.6 %) and K22-20 (96.6 %). However, the digital DNA–DNA genome hybridization value between strain W204 and the closest related strain CPCC 203535 was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H). The predominant cellular fatty acids were iso-C, anteiso-C and C. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W204 (=GDMCC 1.1391=KCTC 49237).

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  • Accepted:
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Keyword(s): meadow soil , Ornithinimicrobium , polyphasic approach and whole-genome sequencing
Funding
This study was supported by the:
  • Fundamental Research Fund for Central Non-Profit Scientific Institution (Award 1610392019006)
    • Principle Award Recipient: Xiubin Ke
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31560023)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31930004)
  • Ministry of Agriculture of China (Award 2019ZX08010-004)
© 2020 The Authors
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2020-11-11
2024-04-24
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Ornithinimicrobium pratense sp. nov., isolated from meadow soil
Int J Syst Evol Microbiol 70, 6450 (2020); https://doi.org/10.1099/ijsem.0.004557
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