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Abstract

A novel actinomycete, designated strain QMT-28, was isolated from rhizosphere soil of collected from Shuangfeng, Hunan Province, PR China. Strain QMT-28 grew well on International Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into loose spiral spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was -diaminopimelic acid and the whole-cell sugars were galactose and glucose. The predominant fatty acids were C 9, summed feature 6 (C 9,12/C a) and C. The polar lipids included diphosphatidylglycerol, hydroxy phospatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine and several unidentified phospholipids. The major menaquinones were MK-9, MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The genome size of strain QMT-28 was about 8.7 Mbp with a G+C content of 71.2 mol%. Phylogenetic analysis showed that the novel strain was closely related to DSM 40451 (99.5 % similarity), NBRC 13450 (98.9 %), NBRC 13414 (98.9 %), I37 (98.9 %) and ZLN234 (98.8 %). However, the average nucleotide identity values, the digital DNA–DNA hybridization values and the multilocus sequence analysis evolutionary distances between this strain and closely related strains showed that it belonged to a distinct species. In addition, these results were also supported by differences in the phenotypic characteristics between QMT-28 and five closely related type strains. Consequently, strain QMT-28 should represent a novel species of the genus , with the suggested name sp. nov. The type strain is QMT-28 (=CICC 24808=JCM 33796).

Funding
This study was supported by the:
  • Xinke Tang , the National Key R and D Program of China , (Award 2017YFE011760)
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/content/journal/ijsem/10.1099/ijsem.0.004555
2020-11-12
2020-12-01
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