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Volume 70, Issue 12

sp. nov., isolated from mountain river sediment Free

Abstract

During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38 was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus by means of a polyphasic taxonomy study. Strain A38 was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained -diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC, 10 methyl C, anteiso-C and iso-C. The predominant menaquinones were MK-10(H) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to DSM 45142 (99.5 %), DSM 43903 (99.4 %), DSM 45555 (99.4 %), DSM 45782 (99.3 %), DSM 45794 (99.3 %), DSM 43813 (99.2 %) and DSM 45246 (99.2 %). The results of OrthoANIu analysis showed the highest similarity to DSM 43026 (96.4 %). However, the 16S rRNA and gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38 as a novel species. On the basis of the results presented here, we propose to classify strain A38 (=LMG 30467=CECT 30034) as the type strain of the novel species sp. nov.

  • Received:
  • Accepted:
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Keyword(s): dDDH , freshwater environment , Micromonospora , OrthoANIu , polyphasic taxonomy and WGS
© 2020 The Authors
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2020-11-11
2024-04-25
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Micromonospora fluminis sp. nov., isolated from mountain river sediment
Int J Syst Evol Microbiol 70, 6428 (2020); https://doi.org/10.1099/ijsem.0.004554
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