1887

Abstract

A novel facultatively anaerobic, nitrate-reducing bacterium, designated MeG22, was isolated from a freshwater lake in Japan. Cells of the strain were straight rods (0.8×2.5–10 µm), motile, and Gram-stain-negative. For growth, the optimum NaCl concentration was 0 % and the optimum temperature was 30 °C. Under anoxic conditions, strain MeG22 reduced nitrate to nitrite. Major cellular fatty acids were C ω6 (13.6 %), C (11.9 %), anteiso-C (10.6 %) and iso-C (10.6 %). The major respiratory quinone was menaquinone-7. The genome sequence of strain MeG22 consists of 5 712 279 bp with a G+C content of 40.3 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family within the phylum . The closest relative of strain MeG22 was strain JAM-BA0302 with a 16S rRNA gene sequence similarity of 90.9 %. On the basis of phylogenetic and phenotypic characterization, , gen. nov., sp. nov., belonging to the family is proposed with the type strain MeG22 (=NBRC 112896=DSM 106262).

Funding
This study was supported by the:
  • Miho Watanabe , Research Fellow of Japan Society for the Promotion Science
  • Manabu Fukui , Japan Society for the Promotion of Science , (Award 22370005)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004551
2020-11-06
2021-02-26
Loading full text...

Full text loading...

References

  1. Huang XF, Liu YJ, Dong JD, Qu L-Y, Zhang Y-Y et al. Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. Int J Syst Evol Microbiol 2014; 64:875–881 [CrossRef][PubMed]
    [Google Scholar]
  2. Iino T, Mori K, Itoh T, Kudo T, Suzuki KI et al. Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:3660–3667 [CrossRef][PubMed]
    [Google Scholar]
  3. ZJ D, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov..
  4. Zhou LY, Yu ZL, Xu W, Mu D-S, Du ZJ. Maribellus luteus gen. nov., sp. nov., a marine bacterium in the family Prolixibacteraceae isolated from coastal seawater. Int J Syst Evol Microbiol 2019; 69:2388–2394 [CrossRef][PubMed]
    [Google Scholar]
  5. Meniscus IRL. A new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Evol Microbiol 1977; 27:38–43
    [Google Scholar]
  6. Holmes DE, Nevin KP, Woodard TL, Peacock AD, Lovley DR. Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. Int J Syst Evol Microbiol 2007; 57:701–707 [CrossRef][PubMed]
    [Google Scholar]
  7. Iino T, Sakamoto M, Ohkuma M. Prolixibacter denitrificans sp. nov., an iron-corroding, facultatively aerobic, nitrate-reducing bacterium isolated from crude oil, and emended descriptions of the genus Prolixibacter and Prolixibacter bellariivorans. Int J Syst Evol Microbiol 2015; 65:2865–2869 [CrossRef][PubMed]
    [Google Scholar]
  8. Sun W, Fu T, Jia C, Fu L, Zhou S et al. Puteibacter caeruleilacunae gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea. Int J Syst Evol Microbiol 2020ijsem003948
    [Google Scholar]
  9. Qu L, Zhu F, Hong X, Gao W, Chen J et al. Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm. Int J Syst Evol Microbiol 2011; 61:2885–2889 [CrossRef][PubMed]
    [Google Scholar]
  10. Liu Q-Q, Li X-L, Rooney AP, Du Z-J, Chen G-J. Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae . Int J Syst Evol Microbiol 2014; 64:3473–3477 [CrossRef][PubMed]
    [Google Scholar]
  11. Wu WJ, Liu Q-Q, Chen GJ, Du ZJ, WJ W, ZJ D. Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment. Int J Syst Evol Microbiol 2015; 65:2260–2264 [CrossRef][PubMed]
    [Google Scholar]
  12. Watanabe T, Miura A, Iwata T, Kojima H, Fukui M. Dominance of Sulfuritalea species in nitrate-depleted water of a stratified freshwater lake and arsenate respiration ability within the genus. Environ Microbiol Rep 2017; 9:522–527 [CrossRef][PubMed]
    [Google Scholar]
  13. Widdel F, Bak F. Gram-negative mesophilic sulfate-reducing bacteria. The Prokaryotes 4, 2nd ed. New York: Springer-Verlag; 1992 pp 3352–3378
    [Google Scholar]
  14. Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 1993; 59:695–700 [CrossRef][PubMed]
    [Google Scholar]
  15. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:1 [CrossRef]
    [Google Scholar]
  16. Arai W, Taniguchi T, Goto S, Moriya Y, Uehara H et al. MAPLE 2.3.0: an improved system for evaluating the functionomes of genomes and metagenomes. Biosci Biotechnol Biochem 2018; 82:1515–1517 [CrossRef][PubMed]
    [Google Scholar]
  17. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [CrossRef][PubMed]
    [Google Scholar]
  19. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  20. Tamaoka J, Katayama-Fujimura Y, Kuraishi H. Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 1983; 54:31–36 [CrossRef]
    [Google Scholar]
  21. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [CrossRef]
    [Google Scholar]
  22. Wang X, Tamiev D, Alagurajan J, DiSpirito AA, Phillips GJ et al. The role of the NADH-dependent nitrite reductase, Nir, from Escherichia coli in fermentative ammonification. Arch Microbiol 2019; 201:519–530 [CrossRef][PubMed]
    [Google Scholar]
  23. Chin KJ, Hahn D, Hengstmann U, Liesack W, Janssen PH. Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms. Appl Environ Microbiol 1999; 65:5042–5049 [CrossRef][PubMed]
    [Google Scholar]
  24. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 2013; 41:D590–D596 [CrossRef][PubMed]
    [Google Scholar]
  25. Takai K, Abe M, Miyazaki M, Koide O, Nunoura T et al. Sunxiuqinia faeciviva sp. nov., a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment. Int J Syst Evol Microbiol 2013; 63:1602–1609 [CrossRef][PubMed]
    [Google Scholar]
  26. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J, Kasai H. Sunxiuqinia rutila sp. nov., a new member of the phylum Bacteroidetes isolated from marine sediment. J Gen Appl Microbiol 2014; 60:28–32 [CrossRef][PubMed]
    [Google Scholar]
  28. Wu W-J, Zhou Y-X, Liu Y, Chen G-J, Du Z-J et al. Mangrovibacterium marinum sp. nov., isolated from a coastal sediment. Antonie van Leeuwenhoek 2015; 107:1583–1589 [CrossRef][PubMed]
    [Google Scholar]
  29. Wang F-Q, Shen Q-Y, Chen G-J, Du Z-J. Mariniphaga sediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2015; 65:2908–2912 [CrossRef][PubMed]
    [Google Scholar]
  30. Du J, Lai Q, Liu Y, Dong C, Xie Y et al. Draconibacterium sediminis sp. nov., isolated from river sediment. Int J Syst Evol Microbiol 2015; 65:2310–2314 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004551
Loading
/content/journal/ijsem/10.1099/ijsem.0.004551
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error