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Volume 70, Issue 12

gen. nov. sp. nov., a novel facultatively anaerobic bacterium isolated from a freshwater lake Free

Abstract

A novel facultatively anaerobic, nitrate-reducing bacterium, designated MeG22, was isolated from a freshwater lake in Japan. Cells of the strain were straight rods (0.8×2.5–10 µm), motile, and Gram-stain-negative. For growth, the optimum NaCl concentration was 0 % and the optimum temperature was 30 °C. Under anoxic conditions, strain MeG22 reduced nitrate to nitrite. Major cellular fatty acids were C ω6 (13.6 %), C (11.9 %), anteiso-C (10.6 %) and iso-C (10.6 %). The major respiratory quinone was menaquinone-7. The genome sequence of strain MeG22 consists of 5 712 279 bp with a G+C content of 40.3 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family within the phylum . The closest relative of strain MeG22 was strain JAM-BA0302 with a 16S rRNA gene sequence similarity of 90.9 %. On the basis of phylogenetic and phenotypic characterization, , gen. nov., sp. nov., belonging to the family is proposed with the type strain MeG22 (=NBRC 112896=DSM 106262).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , facultative anaerobic bacterium and Prolixibacteraceae
Funding
This study was supported by the:
  • Research Fellow of Japan Society for the Promotion Science
    • Principle Award Recipient: Miho Watanabe
  • Japan Society for the Promotion of Science (Award 22370005)
    • Principle Award Recipient: Manabu Fukui
© 2020 The Authors
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2020-11-06
2024-04-20
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Aquipluma nitroreducens gen. nov. sp. nov., a novel facultatively anaerobic bacterium isolated from a freshwater lake
Int J Syst Evol Microbiol 70, 6408 (2020); https://doi.org/10.1099/ijsem.0.004551
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