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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

Genome-based classification of gen. nov., sp. nov., isolated from a hot spring in Turkey, with reclassification of as comb. nov. and as comb. nov. Free

Abstract

A novel Gram-stain-positive, rod-shaped, endospore-forming, motile, aerobic bacterium, designated as P2, was isolated from a hot spring water sample collected from Ilica-Erzurum, Turkey. Phylogenetic analyses based on 16S rRNA gene sequence comparisons affiliated strain P2 with the genus , and the strain showed the highest sequence identity to NBRC 15712 (96.7 %). However, the pairwise sequence comparisons of the 16S rRNA genes revealed that strain P2 shared only 94.7 % sequence identity with subsp. NCIB 3610, indicating that strain P2 might not be a member of the genus . The digital DNA–DNA hybridization and average nucleotide identity values between strain P2 and NBRC 15712 were 19.8 and 74.2 %, respectively. The cell-wall peptidoglycan of strain P2 contained -diaminopimelic acid. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminophospholipid, five unidentified phospholipids and two unidentified lipids while the predominant isoprenoid quinone was MK-7. The major fatty acids were iso-C and iso-C. The draft genome of strain P2 was composed of 82 contigs and found to be 3.5 Mb with 36.1 mol% G+C content. The results of phylogenomic and phenotypic analyses revealed that strain P2 represents a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is P2 (=CECT 9886=DSM 107530=NCCB 100675). Based on the results of the present study, it is also suggested that and should be transferred to this novel genus as comb. nov. and comb. nov., respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Calidifontibacillus erzurumensis , hot spring , new genus , polyphasic taxonomy and thermophilic
Funding
This study was supported by the:
  • Atatürk Üniversitesi
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2020-11-09
2024-04-23
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Genome-based classification of Calidifontibacillus erzurumensis gen. nov., sp. nov., isolated from a hot spring in Turkey, with reclassification of Bacillus azotoformans as Calidifontibacillus azotoformans comb. nov. and Bacillus oryziterrae as Calidifontibacillus oryziterrae comb. nov.
Int J Syst Evol Microbiol 70, 6418 (2020); https://doi.org/10.1099/ijsem.0.004549
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