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Volume 70, Issue 12

sp. nov. and sp. nov., isolated from soil and mineral water Free

Abstract

Two bacterial strains designated as W3-2-3 and HKS04 were isolated from mineral water and a soil sample, respectively, in the Republic of Korea. The 16S rRNA genes of the two strains shared a sequence similarity of 93.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains W3-2-3 and HKS04 formed a distinct lineage within the genus of the family (order ). The closely related species of strain W3-2-3 were (98.9 %), (98.8 %), (98.6 %), (98.5 %), (98.4 %), (98.2 %) and (98.1 %). The closest species of strain HKS04 was (98.7 %). The genome sizes of strains W3-2-3 and HKS04 were 4741198 and 5 120341 bp, respectively. The genomic DNA G+C contents of strains W3-2-3 and HKS04 were 73.3 and 72.1 mol%, respectively. The main fatty acids of strain W3-2-3 were C 6 and iso-C and those of strain HKS04 were iso-C and iso-C H. The main polar lipids of both strains were diphosphatidylglycerol and phosphatidylglycerol and the predominant respiratory quinone was MK-8(H), supporting the affiliation of these strains with the genus . Based on the results of biochemical, chemotaxonomic and phylogenetic analyses, two novel species, W3-2-3 (KACC 21211=LMG 31251) and HKS04 (KACC 18879=LMG 31249), are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Nocardioidaceae , Nocardioides and phylogenetic analysis
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology
    • Principle Award Recipient: Not Applicable
  • National Institute of Biological Resources
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2020-11-13
2024-04-23
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Nocardioides convexus sp. nov. and Nocardioides anomalus sp. nov., isolated from soil and mineral water
Int J Syst Evol Microbiol 70, 6402 (2020); https://doi.org/10.1099/ijsem.0.004547
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