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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 12

gen. nov., sp. nov., from the gastrointestinal tract of the biting midge () larva, belongs to a novel lineage fam. nov., ord. nov. and classis nov. of the phylum Free

Abstract

Strain CS-1, a novel facultative anaerobic bacterium, was isolated from the larval gastrointestinal tract of the biting midge, , a vector of the epizootic haemorrhagic disease virus and the bluetongue virus. Cells were Gram-stain-positive, non-motile, non-spore-forming, pleomorphic rods. Optimal growth occurred at pH 7.5 and 37 °C. The G+C content of the genomic DNA was 38.3 mol%, estimated by using HPLC. The dominant cellular fatty acids were C (45.9 %) and C (26.6 %). The polar lipid profile comprised glycolipids, diphosphatidylglycerol, phospholipids and phosphoglycolipids. Respiratory quinones were not detected. Strain CS-1 had very low 16S rRNA gene similarity to members of the phylum : KM45013 (85 % similarity) and MOL361 (88 % similarity). Phylogenetic analysis based on 16S rRNA, genes, and conserved protein sequences of the whole genome revealed that strain CS-1 was related to members of the classes and within the phylum . Furthermore, average nucleotide identity and digital DNA–DNA hybridization analyses of the whole genome revealed very low sequence similarity to species of and ( KM45013 and sp. H121). These results indicate that strain CS-1 belongs to the phylum and represents a new species of a novel genus, family, order and class. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, we propose the novel taxon gen. nov., sp. nov. with the type strain CS-1 (=CCUG 71726=DSM 106607) within the hereby new proposed novel family fam. nov., new order ord. nov. and new class classis nov. in the phylum .

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Keyword(s): Culicoides , Firmicutes and larvae
© 2020 The Authors
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2020-10-30
2025-04-17
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Culicoidibacter larvae gen. nov., sp. nov., from the gastrointestinal tract of the biting midge (Culicoides sonorensis) larva, belongs to a novel lineage Culicoidibacteraceae fam. nov., Culicoidibacterales ord. nov. and Culicoidibacteria classis nov. of the phylum Firmicutes
Int J Syst Evol Microbiol 70, 6482 (2020); https://doi.org/10.1099/ijsem.0.004543
/content/journal/ijsem/10.1099/ijsem.0.004543
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