1887

Abstract

A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1 was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39′ 04.7′ N 101° 17′ 43.7′′ E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1 belongs to the genus with the greatest sequence similarity to KP01 (97.65 %), DSM27406 (97.58 %), and DHOC24 (97.17 %). The major fatty acids of strain Mgbs1 (>10 %) are iso-C, C 5 and iso-C 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1 has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA–DNA hybridization (DDH) score between strain Mgbs1 and DSM27406 was 15.9 %, while DDH values of strain Mgbs1 against DHOC24 and KP01 were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1 against all three reference strains are 73.2 %. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1 represents a novel species of the genus , for which the name sp. nov. is proposed (=DSM 108835=JCM 33276).

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2020-11-04
2020-12-01
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