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Abstract

A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3, was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0–1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3 clustered with species of the genus and formed a distinct sublineage, showing highest similarities to CC-AFH3 (96.88 %), JC206 (95.78 %) and SUEMI08 (95.78 %). The predominant cellular fatty acids were summed feature 3 (C 6 and/or C 7), summed feature 8 (C 7 and/or C 6) and C. The predominant quinone was ubiquinone 8 (Q-8). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid and two unidentified polar lipids. The polyamine pattern showed the presence of putrescine as the major polyamine, with minor amounts of 2-hydroxyputrescine. The DNA G+C content was 60.1 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain 122213-3 should be classified as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is 122213-3 (=CFCC 14286=LMG 30131).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award NO. 31570603)
    • Principle Award Recipient: Ying-hua Lin
  • National Infrastructure of Microbial Resources (Award NIMR-2019-7)
    • Principle Award Recipient: Chun-gen Piao
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/content/journal/ijsem/10.1099/ijsem.0.004537
2020-11-09
2021-08-02
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