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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

gen. nov., sp. nov., a halotolerant bacterium in the family isolated from a coastal sand dune Free

Abstract

A Gram-stain-negative, non-pigmented, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1492, was isolated from a coastal sand dune and its taxonomic position was examined using a polyphasic approach. Cells of strain CAU 1492 grew optimally at 30 °C, pH 7.0 and in 3 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1492 showed that it formed a distinct lineage within the family as a separate deep branch, with 96.8 % or lower sequence similarity values to representatives of the genera , , , , , , and . Strain CAU 1492 was closely related to DPG-138 (96.8 %), SW-277 (96.7 %), SCM-1 (96.7 %), FF3 (96.6 %) and BB-MW15 (96.4 %) based on 16S rRNA gene sequences. The major cellular fatty acids of strain CAU 1492 were cyclo-C 8 and summed feature 8 (C 7/C 6). The polar lipid pattern was composed of phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid. The strain contained Q-10 as the sole respiratory quinone. The draft genome of strain CAU 1492 was 4.63 Mb with a DNA G+C content of 63.1 mol%. The genome includes 4292 protein-coding genes and a five rRNA operons. On the basis of the phenotypic, chemotaxonomic and genomic data, strain CAU 1492 represents a novel genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is CAU 1492 (=KCTC 62998=NBRC 113696).

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Keyword(s): Arenibacterium halophilum gen. nov., sp. nov. , coastal sand dune and Rhodobacteraceae
Funding
This study was supported by the:
  • Chung-Ang University (Award 2019)
    • Principle Award Recipient: Baek Jihye
  • National Institute of Biological Resources (Award NIBR201902203)
    • Principle Award Recipient: Wonyong Kim
© 2020 The Authors
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2020-10-29
2024-12-12
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Arenibacterium halophilum gen. nov., sp. nov., a halotolerant bacterium in the family Rhodobacteraceae isolated from a coastal sand dune
Int J Syst Evol Microbiol 70, 6323 (2020); https://doi.org/10.1099/ijsem.0.004534
/content/journal/ijsem/10.1099/ijsem.0.004534
/content/journal/ijsem/10.1099/ijsem.0.004534
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