@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004533, author = "Bernard, Kathryn A. and Vachon, Alicia and Pacheco, Ana Luisa and Burdz, Tamara and Wiebe, Deborah and Beniac, Daniel R. and Hiebert, Shannon L. and Booth, Tim and Doyle, Dena Annie and Lawson, Paul and Bernier, Anne-Marie", title = "Pseudoxanthomonas winnipegensis sp. nov., derived from human clinical materials and recovered from cystic fibrosis and other patient types in Canada, and emendation of Pseudoxanthomonas spadix Young et al. 2007", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "12", pages = "6313-6322", doi = "https://doi.org/10.1099/ijsem.0.004533", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004533", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "cystic fibrosis patients", keywords = "winnipegensis", keywords = "emendation of Pseudoxanthomonas spadix", keywords = "clinical isolates", abstract = "Twelve isolates recovered from 10 cystic fibrosis/other patient types and a variety of clinical sources, were referred to Canada's National Microbiology Laboratory over 7 years. These were assignable to the genus Pseudoxanthomonas but were unidentifiable to species level. Patients included five males and five females from two geographically separated provinces, ranging in age from 2 months to 84 years. In contrast, most Pseudoxanthomonas species described to date have been derived from water, plants or contaminated soils. By 16S rRNA gene sequencing, the patient strains had ≥99.4 % similarity to each other but only 97.73–98.29 % to their closest relatives, Pseudoxanthomonas spadix or Pseudoxanthomonas helianthi . Bacteria were studied by whole genome sequencing using average nucleotide identity by Blastn, digital DNA–DNA hybridization, average amino acid identity, core genome and single nucleotide variant analyses, MALDI-TOF, biochemical and cellular fatty acid analyses, and by antimicrobial susceptibility testing. Bacterial structures were assessed using scanning and transmission electron microscopy. Strains were strict aerobes, yellowish-pigmented, oxidative, non-motile, Gram-stain-negative bacilli and generally unable to reduce nitrate. Strains were susceptible to most of the antibiotics tested; some resistance was observed towards carbapenems, several cephems and uniformly to nitrofurantoin. The single taxon group observed by 16S rRNA gene sequencing was supported by whole genome sequencing; genomes ranged in size from 4.36 to 4.73 Mb and had an average G+C content of 69.12 mol%. Based on this study we propose the name Pseudoxanthomonas winnipegensis sp. nov. for this cluster. Pseudoxanthomonas spadix DSM 18855T, acquired for this study, was found to be non-motile phenotypically and by electron microscopy; we therefore propose the emendation of Pseudoxanthomonas spadix Young et al. 2007 to document that observation.", }