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Volume 71, Issue 1

sp. nov., isolated from deep seawater of the Pacific Ocean Free

Abstract

A taxonomic study was carried out on strain C16B3, which was isolated from deep seawater of the Pacific Ocean. The bacterium was Gram-stain-negative, oxidase- and catalase- positive and rod-shaped. Growth was observed at salinities of 0–8.0 % and at temperatures of 10–45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C16B3 belonged to the genus , with the highest sequence similarity to KIS83-12 (97.2 %), followed by MN28 (97.0 %) and the other four species of the genus (94.5 –96.8 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain C16B3 and the type strains of the genus were 74.05−79.48 % and 19.5–22.5 %, respectively. The principal fatty acids (>5 %) were summed feature 8 (C 7/C 6; 20.9 %), iso-C (14.6 %), C 5 (9.4 %), iso-C (8.4 %), summed feature 2 (C 3-OH/iso I-C and C aldehyde; 6.8 %) and C (5.5 %). The G+C content of the chromosomal DNA was 65.37 mol%. The respiratory quinone was determined to be Q-8 (100 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified aminolipids, six unidentified phospholipids and one unidentified polar lipid. The combined genotypic and phenotypic data show that strain C16B3 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain C16B3 (=MCCC 1A04678=KCTC 52314).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep seawater , Pacific Ocean , Solimonas marina sp. nov. and taxonomy
Funding
This study was supported by the:
  • COMRA program (Award No.DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award 16PZP001SF16)
    • Principle Award Recipient: ZongzeShao
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2020-9)
    • Principle Award Recipient: ZongzeShao
© 2021 The Authors
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2020-11-30
2024-12-02
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Solimonas marina sp. nov., isolated from deep seawater of the Pacific Ocean
Int J Syst Evol Microbiol 71, 004528 (2020); https://doi.org/10.1099/ijsem.0.004528
/content/journal/ijsem/10.1099/ijsem.0.004528
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