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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., isolated from soil in the Yellow River Delta wetlands Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1, was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0–15.0 % NaCl, 4–45 °C and pH 6.0–9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1 represented a member of the genus , with the highest sequence similarity to YIM-C238 (97.9 %), followed by (97.1 %) and (96.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between YR1-1 and other closely related type strains of species of the genus were 68.7–86.3% and 17.8–30.9 %. The genome of the strain was 2 899 374 bp in length with 39.8 % DNA G+C content. The predominant fatty acids (>10 %) were iso-C and anteiso-C. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1 represents a novel species within the genus , for which the name sp. nov., is proposed. The type strain is YR1-1 (=KCTC 72794=CGMCC 1.17458).

  • Received:
  • Accepted:
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Keyword(s): novel species , Polyphasic taxonomy , Psychroflexus and Psychroflexus aurantiacus
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070030)
    • Principle Award Recipient: Yue-zhong Li
  • the National Key Research and Development Programs of China (Award 2018YFA0900400 and No. 2018YFA0901704)
    • Principle Award Recipient: Yue-zhong Li
  • the Key Program of Shandong Natural Science Foundation (Award ZR2016QZ002)
    • Principle Award Recipient: Yue-zhong Li
  • the National Natural Science Foundation of China (Award 31670076 and 31471183)
    • Principle Award Recipient: Yue-zhong Li
  • the special national project on investigation of basic resources of China (Award 2019FY100700)
    • Principle Award Recipient: Duo hong Sheng
  • Special investigation on scientific and technological basic resources (Award 2017FY100302)
    • Principle Award Recipient: Yue-zhong Li
© 2020 The Authors
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2020-10-29
2024-12-10
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Psychroflexus aurantiacus sp. nov., isolated from soil in the Yellow River Delta wetlands
Int J Syst Evol Microbiol 70, 6284 (2020); https://doi.org/10.1099/ijsem.0.004527
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