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Abstract

A marine alphaproteobacterium, designated as strain GH3-10, was isolated from the rhizosphere mud of a halophyte () collected at the seashore of Gangwha Island, Republic of Korea. The isolate was found to be Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive, non-motile, short rods and produced orange-coloured colonies. The 16S rRNA gene- and whole genome-based phylogenetic analyses exhibited that strain GH3-10 belonged to the genus and was most closely related to s21-N3 (98.7 % 16S rRNA gene sequence similarity) and KCTC 23554 (98.4 %). The major respiratory quinone was ubiquinone-10. The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unidentified lipid. The major fatty acids were C 7, summed feature 3 (C 7 and/or C 6) and C 7 10-methyl. The DNA G+C content was 61.3 mol% (by genome). Average nucleotide identity and DNA–DNA relatedness values between the isolate and its phylogenetically closest relatives, together with phenotypic distinctness warranted the taxonomic description of a new species. On the basis of data obtained by a polyphasic approach, strain GH3-10 (=KCTC 62379=JCM 32444) represents a novel species of the genus , for which the name sp. nov. is proposed. According to phylogenetic coherence based on 16S rRNA genes and core genomes, it is also proposed that Lee . 2019. and Yoon . 2003 be transferred to comb. nov. and comb. nov., respectively.

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2020-10-23
2021-02-26
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