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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., sp. nov. and sp. nov., three marine bacteria isolated from seawater Free

Abstract

Three Gram-stain-negative, non-motile, rod-shaped strains, designated 72, NH166 and 40DY170, were isolated from seawater samples of the West Pacific Ocean, South China Sea and West Pacific Ocean, respectively. The 16S rRNA gene sequence similarity results revealed that strains 72and NH166 were most closely related to Ar-22, JCM 17757, KCTC 23501, KCTC 22173 and MT-229 with 97.2–98.0% sequence similarity. 16S rRNA gene sequence analysis also indicated that strain 40DY170 was most closely related to DSM 13258, JCM 11811, KCTC 22173 and 501str8 with 97.6–98.1% sequence similarity. The 16S rRNA gene sequence similarity values among strains 72, NH166 and 40DY170 were 96.5–99.2%. Phylogenetic analyses indicated that three new isolates represented three novel species by forming two distinctive lineages within the genus . The DNA G+C contents of strain 72, NH166 and 40DY170 were 43.4, 43.4 and 42.4 mol%, respectively. The average nucleotide identity and DNA–DNA hybridization values between strains 72, NH166, 40DY170 and the reference strains were 76.5–93.5% and 19.2–53.5%, respectively. The sole respiratory quinone in all strains was menaquinone-6. Their major fatty acids were iso-C 3-OH, iso-C and iso-C G. The major polar lipids of strains 72 and NH166 were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The major polar lipids of strain 40DY170 were phosphatidylglycerol, one unidentified phospholipid, one unidentified aminolipid and two unidentified lipids. On the basis of their distinct taxonomic characteristics, the three isolates represent three novel species of the genus , for which the names sp. nov. (type strain 72=KCTC 62229=MCCC 1K03350), sp. nov. (NH166=KCTC 62228=MCCC 1K03449) and sp. nov. (40DY170=KCTC 72200=MCCC 1K03569) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , Muricauda , phylogenetic analysis , seawater and whole genome sequencing
Funding
This study was supported by the:
  • Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources (Award NBHY-2017-P2)
    • Principle Award Recipient: Yuehong Wu
  • the National Natural Science Foundation of China (Award 41876182)
    • Principle Award Recipient: Yuehong Wu
  • the Scientific Research Fund of the Second Institute of Oceanography, MNR (Award JT1702)
    • Principle Award Recipient: Yuehong Wu
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY135-B2-10)
    • Principle Award Recipient: Yuehong Wu
© 2020 The Authors
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2024-04-19
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Muricauda maritima sp. nov., Muricauda aequoris sp. nov. and Muricauda oceanensis sp. nov., three marine bacteria isolated from seawater
Int J Syst Evol Microbiol 70, 6240 (2020); https://doi.org/10.1099/ijsem.0.004522
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