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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., isolated from the Jiulong River Estuary in PR China Free

Abstract

A novel Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive bacterium of the genus , designated strain E2-1, was isolated from surface water of Jiulong River Estuary, PR China. Cells of strain E2-1 grew in medium containing 0.5–12 % NaCl (w/v; optimum, 2–4 %), at 15–45 °C (optimum, 28–33 °C) and at pH 7.0–9.0 (optimum, pH 7.0–8.0). Comparative analyses of the 16S rRNA gene sequence revealed that strain E2-1 had the highest similarity to JLT1210 (97.3 %) and HTCC2597 (97.1 %), and had less than 97.0 % 16S rRNA gene sequence similarity to other type strains within the genus . The DNA G+C content of strain E2-1 was 65.7 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between E2-1 and related type strains were 75.0 and 20.1 % with JLT1210 and 75.6 and 20.4 % with HTCC2597, respectively. The sole isoprenoid quinone was Q-10; the predominant polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and six unidentified lipids; the major cellular fatty acids were C (17.5 %), C cyclo 8 (22.7 %) and summed feature 8 (C 7C 6; 10.1 %). According to the phylogenetic and genotypic results, strain E2-1 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is E2-1 (=MCCC 1K03742=KCTC 72107).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Jiulong River Estuary , Pseudooceanicola , surface water and taxonomy
Funding
This study was supported by the:
  • Key Project of Chinese Ministry of Education (Award 113039A)
    • Principle Award Recipient: Hantao Zhou
  • National Science Fund (Award 31371988)
    • Principle Award Recipient: Hantao Zhou
© 2020 The Authors
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2020-10-15
2024-04-23
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Pseudooceanicola aestuarii sp. nov., isolated from the Jiulong River Estuary in PR China
Int J Syst Evol Microbiol 70, 6220 (2020); https://doi.org/10.1099/ijsem.0.004520
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