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Volume 70, Issue 12

Genome-based reclassification of Sp245 as the type strain of sp. nov Free

Abstract

sp. strain Sp245, originally identified as belonging to , is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. sp. Sp245 and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to Sp7 and CC-Nfb-7. Housekeeping genes revealed that sp. Sp245, BR 12001 and Vi22 form a separate cluster from strain CC-Nfb-7, and a group of strains closely related to Sp7. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between sp. Sp245 and its close relatives to other species type strains, such as Sp7 and CC-Nfb-7 revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of Sp7, CC-Nfb-7 and sp. Sp245, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245 was 68.4–68.6 %. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245 together with the strains Vi22 and BR12001 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Sp245 (=BR 11005=IBPPM 219) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Azospirillum , Azospirillum brasilense Sp7 , Genomic metrics , housekeeping phylogenies and Proteoma- core phylogeny
Funding
This study was supported by the:
  • CAPES
    • Principle Award Recipient: Natalia Ferreira
  • CNPq
    • Principle Award Recipient: Natalia Ferreira
  • Embrapa
    • Principle Award Recipient: Natalia Ferreira
© 2020 The Authors
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2020-10-16
2024-04-20
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Genome-based reclassification of Azospirillum brasilense Sp245 as the type strain of Azospirillum baldaniorum sp. nov
Int J Syst Evol Microbiol 70, 6203 (2020); https://doi.org/10.1099/ijsem.0.004517
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