1887

Abstract

Two Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HMF7605 and HMF7616, were isolated from birch tree, in Yong-in, Republic of Korea. Strains HMF7605 and HMF7616 exhibited the highest 16S rRNA gene sequence similarities of 95.9 and 97.5 % to 17mud1-7, 97.9 % between themselves. The values of average nucleotide identity and DNA–DNA hybridization between strains HMF7605 and HMF7616 were 77.6 and 22.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus within the family . The predominant fatty acids of both strains were iso-C, summed feature 3 (comprising C c and/or C 6c), C 5c and summed feature 4 (comprising iso-C I and/or anteiso-C B). The both strains contained menaquinone-7 as the only isoprenoid quinone. The major polar lipid profiles of the two strains were similar with phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified glycolipid and three unidentified polar lipids. The DNA G+C contents of strains HMF7605 and HMF7616 were 42.0 and 42.8 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, sp. nov. and sp. nov. are proposed. The type strains are HMF7605 (=KCTC 62465=NBRC 113228) and HMF7616 (=KCTC 62466=NBRC 113229), respectively.

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2020-10-16
2021-08-02
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