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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., a bacterium isolated from mangrove sediment Free

Abstract

A Gram-stain-negative, rod-shaped, motile and strictly aerobic bacterium, designated LHK132, was isolated from a mangrove sediment sample collected in Haikou, Hainan Province, PR China. Strain LHK132 was able to grow at temperatures of 10–45 °C, at salinities of 0–7.0 % (w/v) and at pH 6.0–9.0. Catalase and oxidase activities, HS production, urease and methyl red reaction were positive. Indole, nitrate reduction, hydrolysis of gelatin, starch, casein and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were C and summed feature 3 (C 7 and/or C 6). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. According to 16S rRNA gene sequence analysis, strain LHK132 had 98.3, 97.5, 97.4, 97.2 and 97.1% similarities to BD-d46, YQF-1, JA3-B52, IITR-13 and J-MS1, respectively. Phylogenetic analyses indicated that strain LHK132 formed a distinct lineage with BD-d46 within the genus . The average nucleotide identity and digital DNA–DNA hybridization values between strain LHK132 and related species of the genus were well below the thresholds for species delineation. The DNA G+C content was 46.7 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic data, strain LHK132 is considered a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is LHK132 (=KCTC 62580=MCCC 1K03529).

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  • Accepted:
  • Published Online:
Keyword(s): mangrove sediment , Rheinheimera mangrovi and taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Ningbo (Award 2018A610303)
    • Principle Award Recipient: Weiyan Zhang
  • National Natural Science Foundation of China (Award 41776168)
    • Principle Award Recipient: Shan He
  • Open funds of the State Key Laboratory of Agricultural Microbiology (Award AMLKF201905)
    • Principle Award Recipient: Weiyan Zhang
  • State Key Laboratory of Microbial Technology Open Projects Fund (Award M2019-05)
    • Principle Award Recipient: Weiyan Zhang
© 2020 The Authors
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2020-10-14
2024-04-25
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Rheinheimera mangrovi sp. nov., a bacterium isolated from mangrove sediment
Int J Syst Evol Microbiol 70, 6188 (2020); https://doi.org/10.1099/ijsem.0.004513
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