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Volume 70, Issue 12

sp. nov., isolated from a freshwater lake Free

Abstract

A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated strain IMCC25678, was isolated from an artificial freshwater reservoir, Chungju Lake, in the Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC25678 belongs to the genus with ≤98.7 % sequence similarities to species. Whole genome sequencing of strain IMCC25678 revealed a 3.9 Mbp genome size with a DNA G+C content of 42.2 mol%. The IMCC25678 genome shared ≤89.7 % average nucleotide identity and ≤21.4 % digital DNA–DNA hybridization values with closely related species of the genus , indicating that the strain represents a novel species. Summed feature 3 (C 6 and/or C 7), iso-C and iso-C 3-OH were found to be the predominant cellular fatty acid constituents in the strain. The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unidentified phosphoglycolipid, one unidentified sphingolipid and three unidentified polar lipids. Based on the phylogenetic and phenotypic characteristics, strain IMCC25678 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is IMCC25678 (=KACC 19485=NBRC 113130).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): freshwater , genome , novel species , Sphingobacterium , Sphingobacterium chungjuense and taxonomy
Funding
This study was supported by the:
  • Ministry of Education, Science and Technology (Award 2019R1A2B5B02070538)
    • Principle Award Recipient: Jang-Cheon Cho
© 2020 The Authors
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2020-10-15
2024-04-18
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Sphingobacterium chungjuense sp. nov., isolated from a freshwater lake
Int J Syst Evol Microbiol 70, 6126 (2020); https://doi.org/10.1099/ijsem.0.004507
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