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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 11

sp. nov., isolated from Baiyang Lake Free

Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped, brick red-pigmented bacterium, designated R-22-1 c-1, was isolated from water from Baiyang Lake, Hebei Province, PR China. The strain was able to grow at 20–30 °C (optimum, 30 °C) and pH 6–7 (optimum, pH 6) in Reasoner’s 2A medium. 16S rRNA gene sequence and phylogenetic analyses of R-22-1 c-1 revealed closest relationships to MCC P1 (97.8 %), H-1 (97.9 %) and MDT1-10-3 (97.0 %), with other species of the genus showing less than 97.0 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids. The major cellular fatty acids were iso-C, C 6, C 6, anteiso-C, summed feature 3 (iso-C 2-OH and/or C 7 and/or C 6) and summed feature 4 (iso-CI and/or anteiso-CB). The respiratory quinone was MK-7. The draft genome of R-22-1 c-1 was 5.6 Mbp in size, with a G+C content of 50.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain R-22-1 c-1 and related type strains were MCC P1 (77.2 and 21.8 %), H-1 (81.6 and 21.4 %) and 1351 (78.5 and 22.9 %). Based on these phylogenetic, chemotaxonomic and genotypic results, strain R-22-1 c-1 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is R-22-1 c-1 (=CGMCC 1.13570=KCTC 62781).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Baiyang Lake , freshwater and Rufibacter latericius sp. nov.
Funding
This study was supported by the:
  • Beijing Municipal Science & Technology Commission (Award No. Z161100000116042)
    • Principle Award Recipient: yunzhen yang
  • National Natural Science Foudation of China (Award No. 31872632)
    • Principle Award Recipient: yunzhen yang
  • Science and Technology Basic Resources Investigation Project (Award No. 2017FY100300)
    • Principle Award Recipient: yunzhen yang
© 2020 The Authors
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2020-10-05
2024-04-25
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Rufibacter latericius sp. nov., isolated from Baiyang Lake
Int J Syst Evol Microbiol 70, 5943 (2020); https://doi.org/10.1099/ijsem.0.004500
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