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Abstract

An aerobic, non-motile, Gram-stain-negative, pink, convex, coccobacilli-shaped, mesophilic bacterium, designated strain BU-1, was isolated from an urban soil sample from Zibo city, Shandong province, PR China. The strain grew at 20–37 °C (optimum, 30 °C), pH 5–10 (optimum, pH 7) and growth occurred with 0–2 % (w/v) NaCl (optimally with 0.5 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that BU-1 was closely related to members of the genus and had highest 16S rRNA gene sequence similarities with JCM 15073 (97.8 %), JCM 14634 (96.9 %), JCM 15034 (96.5 %), and JCM 17520 (95.9 %). BU-1 also formed a subcluster with JCM 15073 and JCM 15034 in phylogenetic trees based on genomic sequences. The genome size of BU-1 was 5.79 Mb and the DNA G+C content was 71.7 %. ANI, dDDH and AAI values between BU-1 and JCM 15073 were 84.0, 27.2 and 86.7 %, respectively. Furthermore, the genome of BU-1 contained 5446 predicted protein coding genes and 4945 (90.8%) of them had classifiable functions. BU-1 contained Q-10 as the main ubiquinone. The predominant fatty acids (>10 %) were summed feature 3, summed feature 8 and C. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and five unidentified aminolipids. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that strain BU-1 is a representative of a novel species of the genus . Since strain BU-1 can reduce highly toxic selenite [Se(IV)] to low toxicity elemental selenium [Se(0)], the name sp. nov. is proposed. The type strain is BU-1 (=KACC 21750 =GDMCC 1.1776).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31500085)
    • Principle Award Recipient: Hongliang Liu
  • Natural Science Research Project of Hengshui University (Award 2018ZX001)
    • Principle Award Recipient: Shuzhen Wei
  • National Natural Science Foundation of China (Award 81602962)
    • Principle Award Recipient: Feng Sang
  • National Natural Science Foundation of China (Award 31900694)
    • Principle Award Recipient: Yanfang Zhao
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2020-10-09
2021-08-02
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