RT Journal Article SR Electronic(1) A1 Sheu, Shih-Yi A1 Yang, Che-Chia A1 Sheu, Der-Shyan A1 Tsai, Jyh-Ming A1 Chen, Wen-MingYR 2020 T1 Sphingomonas lacunae sp. nov., isolated from a freshwater pond JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 11 SP 5899 OP 5910 DO https://doi.org/10.1099/ijsem.0.004491 PB Microbiology Society, SN 1466-5034, AB A novel bacterial strain, designated CSW-10T, isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed yellow-coloured colonies. Optimal growth occurred at 30 °C, pH 7, and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain CSW-10T formed a phylogenetic lineage in the genus Sphingomonas . The 16S rRNA gene sequence similarity indicated that strain CSW-10T was most closely related to Sphingomonas fonticola TNR-2T (97.6%). Strain CSW-10T showed 69.8–70.7% average nucleotide identity and 19.0–23.0% digital DNA–DNA hybridization identity with the strains of other related Sphingomonas species. The major fatty acids of strain CSW-10T were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C17:1 ω6c. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylcholine, one uncharacterized sphingoglycolipid, five uncharacterized aminophospholipids, one uncharacterized phospholipid and one uncharacterized lipid. The predominant polyamines were homospermidine and spermidine. The major isoprenoid quinone was Q-10. Genomic DNA G+C content of strain CSW-10T was 62.0 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain CSW-10T should represent a novel species of the genus Sphingomonas , for which the name Sphingomonas lacunae sp. nov. is proposed. The type strain is CSW-10T (=BCRC 81190T =LMG 31340T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004491