
Full text loading...
A Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and non-flagellated marine bacterium strain, designated BOM4T, was isolated from a mussel inhabiting the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C, and the optimum temperature was 37 °C. Optimal growth occurred at pH 7.0 and in the presence of 1 % (w/v) NaCl. The predominant isoprenoid quinone of strain BOM4T was identified as menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C15 : 0(43.8 %) and iso-C17 : 0 3-OH (17.5 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene sequence analyses, strain BOM4T was found to be most closely related to Gramella aestuarii JCM 17790T (96.7 %), followed by Gramella flava JLT2011T (96.1 %), Gramella sediminilitoris GHTF-27T (95.6 %) and Gramella gaetbulicola RA5-111T (95.5 %) and with lower sequence similarities (93.7- 95.4 %) to other species of the genus Gramella . Genome relatedness between strain BOM4T and G. aestuarii JCM 17790T was computed using both average nucleotide identity and DNA–DNA hybridization with values of 75.6 and 19.3±2.4 %, respectively. The DNA G+C content of strain BOM4T was 41.4 mol%. On the basis of polyphasic analysis, strain BOM4T was considered to represent a novel species of the genus Gramella , for which the name Gramella bathymodioli sp. nov. is proposed. The type strain is BOM4T (=MCCC 1K03735T=JCM 33424T).