1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 11

sp. nov., isolated from a mussel inhabiting a hydrothermal field in the Okinawa Trough Free

Abstract

A Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and non-flagellated marine bacterium strain, designated BOM4, was isolated from a mussel inhabiting the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C, and the optimum temperature was 37 °C. Optimal growth occurred at pH 7.0 and in the presence of 1 % (w/v) NaCl. The predominant isoprenoid quinone of strain BOM4 was identified as menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C(43.8 %) and iso-C 3-OH (17.5 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene sequence analyses, strain BOM4 was found to be most closely related to JCM 17790 (96.7 %), followed by JLT2011 (96.1 %), GHTF-27 (95.6 %) and RA5-111 (95.5 %) and with lower sequence similarities (93.7- 95.4 %) to other species of the genus . Genome relatedness between strain BOM4 and JCM 17790 was computed using both average nucleotide identity and DNA–DNA hybridization with values of 75.6 and 19.3±2.4 %, respectively. The DNA G+C content of strain BOM4 was 41.4 mol%. On the basis of polyphasic analysis, strain BOM4 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BOM4 (=MCCC 1K03735=JCM 33424).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Gramella bathymodioli , hydrothermal field , mussel and Okinawa Trough
Funding
This study was supported by the:
  • Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (Award 2016ASKJ14)
    • Principle Award Recipient: Xiao-Hua Zhang
  • The Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao) (Award LMEES-YTSP-2018-02-07)
    • Principle Award Recipient: Min Yu
  • National Natural Science Foundation of China (Award U1706208)
    • Principle Award Recipient: Min Yu
  • National Natural Science Foundation of China (Award 41976137)
    • Principle Award Recipient: Min Yu
  • National Key R&D Program of China (Award 2018YFC0310701)
    • Principle Award Recipient: Min Yu
© 2020 The Authors
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2020-09-30
2023-09-24
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Gramella bathymodioli sp. nov., isolated from a mussel inhabiting a hydrothermal field in the Okinawa Trough
Int J Syst Evol Microbiol 70, 5854 (2020); https://doi.org/10.1099/ijsem.0.004488
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