1887

Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterium, designated as strain R-22–3 w-18, was isolated from Baiyangdian Lake in Xiong'an New Area, PR China. Growth was observed at 15–37 °C (optimum, 30 °C) and pH 7.0–10.0 (optimum, pH 8.0). 16S rRNA gene sequence analysis placed the strain in a separate branch in the group, with DSM12111 as its closest phylogenetic relative (98.2 %). Whole-genome comparisons, using average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) analyses, confirmed low genome relatedness to the closely related species [below the recommended thresholds of 95 % (ANI), 95 % (AAI) and 70 % (DDH) for species delineation]. Phenotypic characterization tests could also distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, it is clear that strain R-22–3 w-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-22–3 w-18 (=CGMCC 1.17250=KCTC 72658).

Funding
This study was supported by the:
  • National Natural Science Foudation of China (Award No. 31872632)
    • Principle Award Recipient: Chao Wang
  • Science and Technology Basic Resources Investigation Project (Award No. 2017FY100300)
    • Principle Award Recipient: Jie Feng
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2020-09-30
2021-07-29
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