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Volume 70, Issue 11

sp. nov., isolated from deep-sea sediment of the Western Pacific Ocean Free

Abstract

A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1, was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain O1 showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1–5 % (w/v) NaCl. The predominant menaquinone was MK-8 (H), and anteiso-C and anteiso-C were the major fatty acids. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid. The DNA G+C content of strain O1 was 64.9 mol% and the genome size was 4.17 Mb. Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1 belonged to the genus . The values of average nucleotide identity and DNA–DNA hybridization between strain O1 and its close relatives were well below the thresholds used for the delineation of a new species. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is O1 (=JCM 33845=MCCC 1A16744).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brevibacterium profundi sp. nov. , polyphasic taxonomy and Western Pacific Ocean
Funding
This study was supported by the:
  • National Science and Technology Program during the Twelfth Five-year Plan Period (CN) (Award 201904020029)
    • Principle Award Recipient: Rui Zhang
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2019011)
    • Principle Award Recipient: Gaiyun Zhang
  • China Ocean Mineral Resources R&D Association (COMRA) Program (Award DY135-B2-01)
    • Principle Award Recipient: Gaiyun Zhang
© 2020 The Authors
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2020-09-23
2024-04-25
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Brevibacterium profundi sp. nov., isolated from deep-sea sediment of the Western Pacific Ocean
Int J Syst Evol Microbiol 70, 5818 (2020); https://doi.org/10.1099/ijsem.0.004483
/content/journal/ijsem/10.1099/ijsem.0.004483
/content/journal/ijsem/10.1099/ijsem.0.004483
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