1887

Abstract

As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species. The isolates were Gram-staining-positive, coccoid, facultatively anaerobic, non-motile, catalase-negative and oxidase-negative. Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus , with and as closest relatives. Results indicated that they represented two different novel species with two strains, (TMW 2.1612/TMW 2.1613 and TMW 2.1615/TMW 2.1614), respectively. The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation. The 16S rRNA gene sequences of TMW 2.1612 and TMW 2.1615 had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of , respectively. However, the ANIb value between the respective type strains TMW 2.1612 and TMW 2.1615, and the type strain of was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612 and TMW 2.1615. The DDH estimate value between the respective type strain TMW 2.1612 and TMW 2.1615 and the most closely related type strain of was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612 and TMW 2.1615. Peptidoglycan type of strain TMW 2.1612 is Lys–Thr–Ala and major fatty acids are summed feature 8 and C. Peptidoglycan type of strain TMW 2.1615 is Lys–Ala and major fatty acids are C, Ccyclo ω8 and summed feature 8. On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus , for which the names sp. nov and sp. nov are proposed. The designated respective type strains are TMW 2.1612 (=DSM 111016 =CECT 30115) and TWM 2.1615 (=DSM 111017 =CECT 30116).

Funding
This study was supported by the:
  • Not Applicable , Bundesministerium für Wirtschaft und Energie , (Award 17803N)
  • Not Applicable , Bundesanstalt für Landwirtschaft und Ernährung , (Award BLE 281A105116)
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2020-09-30
2020-11-25
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