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Abstract

A novel bacterial strain of the family ‘’ was isolated from mangrove of Tielu Harbour, Hainan, PR China. Strain S-15 was a Gram-stain-negative, short-rod-shaped, yellow-pigmented that could grow at 10–42 °C (optimum, 26–35 °C), at pH 5.0–9.0 (optimum, pH 5.5) and in 0.5–10.0 % w/v sea salt (optimum, 3.5–4.0 %). Cells of strain S-15 were 0.9–1.4 µm long, 0.8–0.9 µm wide, catalase-positive and oxidase-positive. Colonies on modified marine agar 2216 were 0.5–2.0 mm in diameter after incubation for 72 h at 28 °C. Analysis of 16S rRNA gene sequences revealed that strain S-15 was most closely related to ANORD5 (89.8 %). The major respiratory quinone of strain S-15 was menaquinone MK-7, and the dominant fatty acids were C iso, C iso G and C iso 3-OH. The major polar lipids were two unidentified aminolipids, phosphatidylethanolamine and six unidentified lipids. Analyses showed that the genome size was 3.52 Mb and the DNA G+C content was 35.6 mol%, which were higher than ANORD5 with 2.92 Mb genome size and 31.0 mol% G+C content, respectively. Based on morphological, physiological and phylogenetic data, strain S-15 is considered a type strain of a new species and a new genus of the family ‘’ for which the name gen. nov., sp. nov. is proposed. The type strain of is S-15 (=MCCC 1K03817=JCM 33804).

Funding
This study was supported by the:
  • Jing Zhao , Scientific Research Project of Xiamen Southern Ocean Center, China , (Award 17GZP007NF03)
  • Jing Zhao , Scientific Research Project of the National Natural Science Foundation of China , (Award 41876183)
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/content/journal/ijsem/10.1099/ijsem.0.004480
2020-09-23
2020-10-20
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References

  1. Bowman JP. Out from the shadows - resolution of the taxonomy of the family Cryomorphaceae. Front Microbiol 2020; 11:795 [CrossRef][PubMed]
    [Google Scholar]
  2. Wiese J, Saha M, Wenzel-Storjohann A, Weinberger F, Schmaljohann R et al. Vicingus serpentipes gen. nov., sp. nov., a new member of the Flavobacteriales from the North Sea. Int J Syst Evol Microbiol 2018; 68:333–340 [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  4. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  5. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [CrossRef][PubMed]
    [Google Scholar]
  6. Fothergill AW. Antifungal susceptibility testing: clinical laboratory and standards Institute (CLSI) methods. interactions of yeasts, moulds, and antifungal agents. Humana Press 201265–74
    [Google Scholar]
  7. He YQ, Chen RW, Li C, Shi SB, Cui LQ et al. Actinomarinicola tropica gen. nov. sp. nov., a new marine actinobacterium of the family Iamiaceae, isolated from South China Sea sediment environments. Int J Syst Evol Microbiol 2020; 70:3852–3858 [CrossRef][PubMed]
    [Google Scholar]
  8. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1988; 19:1–67
    [Google Scholar]
  9. Liu Q-Q, Li X-L, Rooney AP, Du Z-J, Chen G-J. Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. Int J Syst Evol Microbiol 2014; 64:3473–3477 [CrossRef][PubMed]
    [Google Scholar]
  10. Zhang H, Han J-R, Shi M-J, Du Z-J, Chen G-J. Brumimicrobium aurantiacum sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2017; 67:3256–3260 [CrossRef][PubMed]
    [Google Scholar]
  11. Altschul S. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [CrossRef]
    [Google Scholar]
  12. Aiyar A. The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment. Methods Mol Biol 2000; 132:221–241 [CrossRef][PubMed]
    [Google Scholar]
  13. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  15. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1991; 9:945–967
    [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 2004; 101:11030–11035 [CrossRef][PubMed]
    [Google Scholar]
  18. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  20. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Del, USA: MIDI, Newark; 1990
    [Google Scholar]
  21. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [CrossRef]
    [Google Scholar]
  22. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [CrossRef]
    [Google Scholar]
  23. Lee I, Chalita M, Ha S-M, Na S-I, Yoon S-H et al. ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences. Int J Syst Evol Microbiol 2017; 67:2053–2057 [CrossRef][PubMed]
    [Google Scholar]
  24. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [CrossRef][PubMed]
    [Google Scholar]
  25. YX Y, Huang Y, Wang JH. The application of molecular biology technology in polyphasic taxonomy of environmental microorganisms. J Cent South Univ T 2004
    [Google Scholar]
  26. Yoon SH, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  27. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [CrossRef][PubMed]
    [Google Scholar]
  28. Li L, Stoeckert CJ, Roos DS, Christian JS, David SR. OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res 2003; 13:2178–2189 [CrossRef][PubMed]
    [Google Scholar]
  29. Edgar RC. Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
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