1887

Abstract

The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite , showed morphological, physiological and chemotaxonomic properties typical of the genus . Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5 was DSM 42102 (98.87 %). DNA–DNA hybridization experiments between strain RB5 and DSM 42102 resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5 contained -diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H) and MK-9(H)]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C) 12-methyltetradecanoic acid (anteiso-C), 13-methyltetradecanoic acid (iso-C) and 14-methylpentadecanoic acid (iso-C). Here, we propose a novel species of the genus with the type strain RB5 (=VKM Ac-2839=NRRL B65539).

Funding
This study was supported by the:
  • Michael Poulsen , Villum Fonden , (Award VKR10101)
  • Christine Beemelmanns , Deutsche Forschungsgemeinschaft , (Award CRC 1127 (A6) and BE-4799/3-1)
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2020-09-24
2020-10-25
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