1887

Abstract

A Gram-stain-positive, non-motile, aerobic, rod-shaped, golden yellow-pigmented actinobacterium, designated strain YIM 123512, was isolated from soil sampled at Gaoligong Mountain, Yunnan Province, PR China. The strain grew at 10–40 °C (optimum, 28 °C), with 0–3.0 % (w/v) NaCl (optimum, 0–1.0 %) and at pH 5.0–11.0 (optimum, pH 7.0). The major menaquinone was MK-8(H) and the major cellular fatty acids (>10 %) were identified as iso-C and C 8. Strain YIM 123512 had -2, 6-diaminopimelic acid as the diagnostic cell-wall diamino acid. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids and one unknown lipid. The genomic DNA G+C content of strain YIM 123512 was 72.7 mol% based on its draft genome sequence. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 123512 appeared to be most closely related to KCTC 19274 (97.7 % sequence similarity) and to belong to the genus . The sequence similarity values of strain YIM 123512 to other currently described type strains of the genus were less than 97.0 %. Based on the draft genome sequence, the novel strain showed an average nucleotide identity value of 80.2 % and a digital DNA–DNA hybridization value of 23.1 % with the reference strain KCTC 19274. The results of the polyphasic taxonomic study including phenotypic, chemotaxonomic and phylogenetic analyses show that strain YIM 123512 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 123512 (=KCTC 49303=CGMCC 4.7628).

Funding
This study was supported by the:
  • National Major Science and Technology Projects of China (Award (AA18242026))
    • Principle Award Recipient: Ming-Guo Jiang
  • National Natural Science Foundation of China (Award (31460005))
    • Principle Award Recipient: Yi Jiang
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2020-09-18
2021-08-02
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